Branched PEG Maleimide is a high-performance bioconjugation reagent featuring a branched PEG structure and a maleimide group. Its design supports efficient conjugation with thiol-containing molecules while enhancing solubility, stability, and drug-to-antibody ratios (DAR). This makes it ideal for ADC synthesis, drug delivery systems, and surface functionalization applications.
Branched PEG Maleimide
Branched PEG Maleimide: Advanced Reagent for Efficient Bioconjugation
Product Overview
Branched PEG Maleimide is a cutting-edge reagent designed for precise bioconjugation and surface functionalization. Featuring a branched polyethylene glycol (PEG) structure and a maleimide group, this versatile compound facilitates the conjugation of biomolecules and hydrophobic drugs with high efficiency.
Key Features
- Branched PEG Structure: The multi-arm PEG backbone enhances solubility and flexibility, making it ideal for conjugating hydrophobic organic molecules, such as maytansinoids, to biomolecules. The branched design supports higher drug-to-antibody ratios (DAR) compared to traditional hydrophobic linkers like SPDB and SMCC, without causing aggregation or compromising the affinity of the resulting conjugate.
- Maleimide Group: Enables specific and efficient coupling with thiol-containing molecules through Michael addition, ensuring precise and stable conjugation.
- Enhanced Stability: The PEG structure reduces non-specific interactions and enhances the stability and solubility of the conjugate.
Applications
- Bioconjugation: Ideal for attaching hydrophobic organic drugs, such as maytansinoids, to peptides, proteins, and antibodies. The branched PEG structure allows for higher DAR while maintaining stability and affinity.
- Surface Functionalization: Effective for modifying surfaces of materials, such as polymers and nanoparticles, to enhance their reactivity and functionality.
- Drug Delivery Systems: Useful in developing targeted drug delivery systems by facilitating the conjugation of therapeutic agents to delivery vehicles without triggering aggregation.
Benefits
- High DAR Capability: The branched PEG linker enables conjugation at higher drug-to-antibody ratios than traditional linkers, enhancing therapeutic efficacy while avoiding aggregation.
- Efficient Conjugation: The maleimide group provides high specificity and efficiency in coupling with thiol-containing molecules.
- Improved Solubility: The multi-arm PEG structure enhances solubility and reduces non-specific binding, contributing to more accurate and reliable experimental outcomes.
Conclusion
Branched PEG Maleimide is a crucial reagent for researchers developing advanced bioconjugation and drug delivery systems. Its ability to conjugate hydrophobic drugs at higher DAR without loss of affinity or triggering aggregation sets it apart from traditional linkers. With its versatile PEG structure and efficient maleimide coupling, this reagent offers superior performance for precise and stable conjugate creation in various scientific and industrial applications.
Ref:
Cahn D, Duncan GA. High-Density Branched PEGylation for Nanoparticle Drug Delivery. Cell Mol Bioeng. 2022 Jul 5;15(5):355-366. doi: 10.1007/s12195-022-00727-x. PMID: 36444352; PMCID: PMC9700551.