Cyanine 7.5 (Cy7.5) is a near-infrared fluorescent dye used in biochemistry and molecular biology for labeling proteins and nucleic acids. It is ideal for multiplexed imaging, flow cytometry, and fluorescence microscopy, offering bright fluorescence, high stability, and minimal background interference.
Cyanine7.5
Cyanine 7.5 (Cy7.5) is a near-infrared fluorescent dye used for labeling proteins and nucleic acids in biochemistry and molecular biology. Often paired with other dyes like Cy3 and Cy5, Cy7.5 enables simultaneous detection of multiple targets in a single experiment, making it highly valuable for multiplexed imaging.
Key Features of Cyanine 7.5 (Cy7.5) Dye:
- Spectral Properties:
- Absorption Maximum: ~788 nm
- Emission Maximum: ~808 nm
- Stokes Shift: ~20 nm
- High Quantum Yield: Provides bright, efficient fluorescence.
- High Photostability: Resists photobleaching, maintaining consistent signals during long-term imaging.
Applications of Cyanine 7.5 (Cy7.5) Dye:
- In Vivo Imaging: Offers deep tissue imaging with minimal background interference.
- Fluorescence Microscopy: Enables clear imaging of cellular structures and processes.
- Flow Cytometry: Provides distinct and reliable signals for cell sorting and analysis.
- Bioanalytical Assays: Effective for detecting and analyzing multiple biomolecular targets in complex assays.
Cy7.5’s bright NIR fluorescence, compatibility with other dyes, and excellent stability make it an ideal choice for advanced research applications in imaging and molecular labeling.