Sulfo Cyanine3 Dye

Sulfo-Cyanine3 Dye: Spectral Properties and Applications

Sulfo-Cyanine3 (Sulfo-Cy3) is a water-soluble, orange-red fluorescent dye widely used in bioconjugation, imaging, and labeling applications. Its enhanced hydrophilicity due to sulfonate groups allows it to perform efficiently in aqueous environments, making it an ideal choice for labeling proteins, nucleic acids, and other biomolecules in biological and biomedical research.

Spectral Properties of Sulfo-Cyanine3 Dye:

• Absorption Maximum: ~554 nm
• Emission Maximum: ~568 nm
• Stokes Shift: ~14 nm
• High Quantum Yield: Provides bright, efficient fluorescence.
• Enhanced Water Solubility: Performs well in aqueous solutions due to sulfonate groups.

Applications of Sulfo-Cyanine3 Dye:

• Fluorescence Microscopy: Ideal for clear imaging of cellular structures and processes.
• Flow Cytometry: Provides distinct signals for precise cell sorting and analysis.
• Bioconjugation: Efficiently labels proteins, antibodies, and nucleic acids for various assays.
• Molecular Probes: Effective for studying biomolecular interactions in bioanalytical assays.

Sulfo-Cy3’s bright fluorescence, excellent water solubility, and strong signal make it a versatile and reliable dye for researchers needing high-performance labeling in complex biological systems.