Cyanine 3.5 (Cy3.5) dye is an orange-red fluorescent dye with absorption at ~581 nm and emission at ~596 nm. It offers bright fluorescence, high photostability, and efficient labeling, making it ideal for applications like fluorescence microscopy, flow cytometry, and protein detection in Western blotting.
Cyanine3.5 Dye
Cyanine 3.5 Dye: Spectral Properties and Applications
Cyanine 3.5 (Cy3.5) dye is a bright orange-red fluorescent dye known for its excellent photostability, high quantum yield, and sharp spectral characteristics. It is widely used in fluorescence imaging, flow cytometry, and other fluorescence-based assays, providing strong and reliable signals for various biological and chemical research applications.
Spectral Properties of Cyanine 3.5 Dye:
- Absorption Maximum: ~581 nm
- Emission Maximum: ~596 nm
- Stokes Shift: ~15 nm
- High Quantum Yield: Delivers efficient fluorescence, making it ideal for sensitive detection.
- Photostability: Resists photobleaching, ensuring consistent fluorescence during prolonged experiments.
- Sharp Spectral Peaks: Narrow absorption and emission spectra reduce overlap, enhancing clarity in multiplex assays.
Applications of Cyanine 3.5 Dye:
- Fluorescence Microscopy: Ideal for high-resolution imaging of cellular structures and processes.
- Flow Cytometry: Provides bright, distinct signals for cell sorting and analysis.
- Molecular Probes: Effective for labeling proteins, nucleic acids, and other biomolecules in research and diagnostics.
- Western Blotting and Immunoassays: Enhances detection and quantification of proteins and antibodies.
Cyanine 3.5 dye’s bright fluorescence, high stability, and precise spectral properties make it an excellent choice for researchers seeking reliable fluorescent labeling in advanced fluorescence-based studies.