DMT-Biotin-C6 Phosphoramidite
This product is a biotinylated DNA/RNA synthesis reagent featuring a dimethoxytrityl (DMT) protecting group, a C6 spacer, and a phosphoramidite functionality. It specifically enables efficient incorporation of biotin into oligonucleotides during automated solid-phase synthesis.
Key Features and Benefits
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Biotin Moiety: Provides high-affinity binding to streptavidin and avidin.
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C6 Spacer: Improves accessibility of biotin for downstream binding assays.
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DMT Protection: Ensures controlled oligonucleotide synthesis.
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Phosphoramidite Chemistry: Compatible with automated DNA/RNA synthesizers.
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High Purity: Guarantees reproducibility in oligo labeling workflows.
Applications
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Oligonucleotide Synthesis: Introduces biotin into DNA or RNA during solid-phase synthesis.
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Bioconjugation: Facilitates streptavidin-based detection and purification.
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Probe Development: Builds biotinylated oligonucleotide probes for imaging and diagnostics.
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Molecular Biology: Used in pull-down assays, ELISA, and nucleic acid capture.
Storage and Handling
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Storage: Keep at –20 °C in a sealed, light- and moisture-protected container.
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Handling: Use under inert atmosphere; avoid prolonged exposure to moisture.
This product combines reliable phosphoramidite chemistry with biotin labeling, specifically offering a robust tool for oligonucleotide synthesis, molecular biology, and probe development.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
