PLLA-PEG-Maleimide (PLLA-PEG-MAL), MW x 1K, n 2K provides an amphiphilic, biodegradable block copolymer for drug delivery, bioconjugation, and nanoparticle research. It combines a 1 kDa poly(L-lactic acid) (PLLA) segment with a 2 kDa hydrophilic polyethylene glycol (PEG) chain terminated with a reactive maleimide (MAL) group. This PEG-rich structure supports self-assembled nanocarrier development and thiol-selective conjugation of peptides, proteins, and targeting ligands.
Key Features and Benefits
- 1 kDa PLLA Block: Provides a biodegradable hydrophobic polyester segment specifically for nanoparticle formation and drug encapsulation research.
- 2 kDa PEG Block: Introduces a hydrophilic polymer chain that specifically supports aqueous compatibility, surface hydration, and colloidal stabilization.
- Reactive Maleimide Functionality: Enables covalent attachment to thiol-containing molecules through Michael addition chemistry.
- Thiol-Selective Conjugation: Supports functionalization with cysteine-containing peptides, proteins, and other sulfhydryl-bearing compounds.
- Amphiphilic Block Copolymer: Combines hydrophobic PLLA and hydrophilic PEG segments to specifically support self-assembly in suitable aqueous formulations.
- Biodegradable Polyester Segment: Contains hydrolytically degradable ester bonds for investigating polymer degradation and controlled-release behavior.
- PEG-Rich Composition: Features a 2:1 PEG-to-PLLA nominal molecular weight ratio specifically for studying hydrated nanocarrier surfaces and polymer assembly.
- Surface Functionalization Capability: Provides terminal maleimide groups specifically for introducing targeting ligands and other functional components.
- Versatile Nanocarrier Design: Supports preparation of functionalized polymeric micelles, nanoparticles, and biodegradable delivery systems.
Applications
- Polymeric Nanoparticle Development: Supports preparation of amphiphilic nanoparticles with hydrophobic PLLA domains and hydrophilic PEG surfaces.
- Targeted Drug Delivery Research: Enables attachment of thiol-containing targeting ligands to polymeric carriers for receptor-targeting studies.
- Peptide Bioconjugation: Supports covalent conjugation of cysteine-containing peptides through maleimide-thiol coupling.
- Protein Functionalization: Enables attachment of compatible thiol-bearing proteins to PEG-functionalized polymer structures.
- Polymeric Micelle Research: Supports investigation of self-assembly, micelle stability, and hydrophobic compound encapsulation.
- Controlled Drug Release: Enables evaluation of drug release behavior associated with biodegradable PLLA-containing nanocarriers.
- Nanoparticle Surface Modification: Supports introduction of functional molecules onto maleimide-bearing PEG surfaces.
- Biomaterials Research: Enables development of biodegradable polymer constructs for studying molecular interactions and surface properties.
- Polymer Characterization: Supports investigation of block composition, particle size, degradation behavior, and conjugation efficiency.
Storage and Handling
- Storage: Store at −20°C in a dry, tightly sealed container under an inert atmosphere when possible, unless otherwise specified. Protect from moisture, excessive heat, and prolonged exposure to light to preserve polymer integrity and maleimide reactivity.
- Handling: Handle under dry conditions and dissolve in a compatible organic solvent, such as DMSO or DMF, according to formulation requirements. For nanoparticle preparation, select an appropriate solvent exchange, dialysis, or nanoprecipitation method based on polymer solubility and the intended formulation.
For thiol conjugation, react the maleimide group with a suitable sulfhydryl-containing molecule, typically under mildly acidic to neutral aqueous conditions around pH 6.5–7.5. Avoid excess thiol-containing reducing agents and prolonged exposure to alkaline conditions, which can reduce maleimide coupling efficiency. Optimize reaction conditions to minimize competing side reactions and preserve biomolecule activity.
PLLA-PEG-Maleimide (PLLA-PEG-MAL), MW x 1K, n 2K combines a biodegradable PLLA segment with a hydrophilic PEG chain and reactive maleimide functionality and specifically supports targeted nanoparticle development, peptide and protein conjugation, polymeric micelle formation, controlled-release studies, and advanced biomaterials research.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.