LumiTracker® Mito JC-1, 47729-63-5; 3520-43-2 is a fluorescent dye used to measure mitochondrial membrane potential in live cells. It changes color based on membrane potential, emitting green fluorescence (~530 nm) as monomers in depolarized mitochondria and red fluorescence (~590 nm) as J-aggregates in polarized, healthy mitochondria. This color shift makes JC-1 a reliable indicator for studying cell health, apoptosis, and mitochondrial function.
Key Features:
- Dual Fluorescence: Emits green in its monomeric form and red when forming J-aggregates, enabling easy detection of mitochondrial health.
- Membrane Potential Sensitivity: Accumulates in mitochondria depending on membrane potential, distinguishing between healthy and damaged cells.
- Ratiometric Analysis: Allows precise measurement of mitochondrial membrane potential by comparing red-to-green fluorescence ratios.
LumiTracker® Mito JC-1 Applications:
- Mitochondrial Health Studies: Monitors changes in mitochondrial activity during apoptosis or cell stress.
- Cell Viability Assays: Evaluates cell health and energy metabolism.
- Apoptosis Detection: Identifies early signs of programmed cell death by tracking mitochondrial depolarization.
How to Use:
- Storage: Keep at -20°C, protected from light and moisture, for long-term stability.
- Solubility: Dissolves in DMSO, making it compatible with various experimental setups.
LumiTracker® Mito JC-1 is a powerful tool for researchers studying mitochondrial function, energy metabolism, and cellular health.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
