ssGreen RNA Gel Staining (CAS 172827-25-7) is a highly sensitive fluorescent dye for detecting RNA and single-stranded DNA (ssDNA) in agarose and polyacrylamide gels. It has a fluorescence yield significantly higher than ethidium bromide, making it ideal for low-abundance nucleic acid detection.
Key Features:
- High Sensitivity: Detects RNA and ssDNA with superior sensitivity.
- Fluorescence: Excitation at 497 nm; emission at 520 nm.
- Compatibility: Works with denaturing gels and is compatible with Northern blot analysis.
- Ease of Use: Requires no pre-removal of denaturants like formaldehyde or urea before staining.
Applications:
- RNA Visualization: Stains RNA in formaldehyde agarose gels for clear band detection.
- ssDNA Detection: Identifies single-stranded DNA in various gel matrices.
- Northern Blotting: Allows RNA transfer and hybridization without interference.
This dye offers a safer, more efficient alternative to ethidium bromide for molecular biology research.
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