PODi-1 (CAS 169454-15-3), also known as POPO®-1, is a blue fluorescent nucleic acid stain. It is nonfluorescent until bound to double-stranded DNA (dsDNA), where it exhibits a significant fluorescence enhancement.
Key Features:
- Excitation Maximum: 433 nm
- Emission Maximum: 457 nm
- Cell Impermeable: Stains only dead or permeabilized cells.
- High Affinity for Nucleic Acids: Produces bright fluorescence upon binding.
Applications:
- Fluorescence Microscopy: Nuclear and chromosome counterstaining in multicolor experiments.
- Flow Cytometry: Differentiates live and dead cells.
- Microarray Analysis: Stains nucleic acids for visualization.
PODi-1 provides bright fluorescence and minimal background, making it suitable for various fluorescence-based studies.
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