Hoechst 33258 (bisbenzimide, HOE 33258) is a blue-emitting, cell-permeable fluorescent dye widely used for DNA staining in cells and tissues. It binds strongly to the minor groove of A-T-rich regions in double-stranded DNA, enhancing fluorescence and enabling imaging and quantification in molecular biology.
Key Features:
- Fluorescence Properties:
- Excitation: ~352 nm
- Emission: ~461 nm (blue fluorescence)
- Significant Stokes shift, suitable for multicolor labeling.
- DNA Binding:
- Preferentially binds A-T-rich DNA regions; fluorescence intensity increases with pH.
- Unbound dye fluoresces green (510–540 nm) at high concentrations or inadequate washing.
- Cell Permeability:
- Penetrates live cells
- Less toxic than DAPI, ensuring higher cell viability.
Hoechst 33258 Applications:
- DNA Visualization: Fluorescence microscopy for chromosomes and nuclei in live or fixed cells.
- Cell Cycle Analysis: Flow cytometry for studying cell division and sorting.
- Apoptosis Detection: Identifies condensed nuclei in apoptotic cells.
- BrdU Quenching: Used to study cell-cycle progression as BrdU integration disrupts Hoechst binding.
Storage and Handling:
- Store at -20°C, protected from light.
- Use in aqueous buffers (e.g., PBS) or organic solvents like DMSO.
Hoechst 33258 is an essential tool in cell biology and genetics for precise DNA-specific staining and fluorescence-based analyses.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
