DSP (Dithiobis[succinimidyl propionate]), CAS:57757-57-0, is a homo-bifunctional crosslinker commonly used in protein crosslinking and bioconjugation. It contains two NHS (N-hydroxysuccinimide) ester groups and a disulfide bridge, making it reactive with primary amines in proteins or peptides, such as those found in lysine residues.
Key Features:
- NHS Ester Groups: These groups react with amines to form stable amide bonds, enabling crosslinking between proteins or other molecules.
- Disulfide Linker: The disulfide bond can be cleaved under reducing conditions, making DSP a cleavable crosslinker that allows for reversible protein modifications.
Applications:
- Protein Crosslinking: DSP is widely used to covalently link proteins or peptides, making it useful in structural biology, protein interaction studies, and immunoassays.
- Bioconjugation: It is applied in the conjugation of biomolecules, enabling the development of antibody-drug conjugates and other targeted therapies.
- Reversible Crosslinking: Due to its disulfide bond, DSP allows for temporary crosslinking that can be reversed using reducing agents like DTT or TCEP.
Benefits:
- Reversible Modifications: DSP enables temporary crosslinking, useful in experiments where removal of the crosslinker is desired after analysis.
- Efficient Conjugation: The NHS esters ensure rapid and efficient conjugation with primary amines, creating stable bonds in a variety of biological applications.
DSP Crosslinker is a versatile reagent in biotechnology and pharmaceutical research, particularly for applications requiring controlled and reversible crosslinking of proteins or other biomolecules.
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