N-(DBCO-PEG4)-N-Biotin-PEG4-NHS
This product is a high-purity heterobifunctional linker with a dibenzocyclooctyne (DBCO) group for rapid, copper-free strain-promoted azide–alkyne cycloaddition (SPAAC), a biotin for high-affinity streptavidin/avidin binding, and an NHS ester for efficient amine coupling. The dual PEG4 spacers specifically enhance solubility, flexibility, and reduce steric hindrance, enabling high-yield, orthogonal bioconjugations in both aqueous and organic systems. This reagent is especially ideal for dual-labeling strategies, biomolecule capture, and targeted delivery applications.
Key Features and Benefits
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DBCO Group: Enables fast, catalyst-free SPAAC click chemistry with azides.
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Biotin Moiety: Provides strong, specific binding to streptavidin or avidin.
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NHS Ester: Couples efficiently with primary amines to form stable amide bonds.
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Dual PEG4 Spacers: Improve solubility, flexibility, and accessibility of reactive groups.
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High Purity: Supplied at >95% purity for reproducible performance.
Applications
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Orthogonal dual labeling of biomolecules.
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Streptavidin-based capture and purification.
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Protein, peptide, and antibody conjugation.
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Development of multifunctional probes and diagnostics.
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Nanoparticle and surface functionalization.
Storage and Handling
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Storage: Store at –20 °C, dry, and protected from light.
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Handling: Use anhydrous solvents such as DMF or DMSO; avoid moisture to preserve NHS ester reactivity.
This product combines copper-free click chemistry, streptavidin binding, and amine coupling in one linker, with PEG4 spacers specifically for optimal solubility and conjugation efficiency.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
