Propargyl-PEG-Mal (Alkyne-PEG-maleimide)

Propargyl-PEG-Mal is an Alkyne-PEG-Maleimide reagent designed for bioconjugation applications. It features an alkyne group, a polyethylene glycol (PEG) spacer, and a maleimide group.

The maleimide group reacts specifically with thiol groups (-SH) on target biomolecules (e.g., proteins, peptides), forming a stable thioether bond in aqueous solution at pH 6.5-7.5. The alkyne group allows for subsequent “click chemistry” reactions, typically with azide-containing compounds, facilitating versatile labeling or tagging. The PEG moiety provides water solubility, biocompatibility and flexibility.

Alkyne-PEG-Maleimide offers several advantages, such as:

Selective Conjugation – Targets thiol groups with high specificity,

Enhanced Solubility – PEG spacer improves solubility and reduces steric hindrance, enhancing conjugation efficiency

Versatility – Enables further modifications through click chemistry.