APDye Fluor 680 is a bright, photostable near-infrared dye. It matches Alexa Fluor® 680 and IRDye® 680RD. Water-soluble and pH-stable, it works well for small animal imaging, Western blotting, ELISA, and flow cytometry. It offers high brightness and low background interference.
APDye Fluor 680
APDye Fluor 680 is a bright, photostable near-infrared dye equivalent to Alexa Fluor® 680 and IRDye® 680RD. It is water-soluble and pH-insensitive (pH 4-10), emitting at ~700 nm, ideal for small animal imaging and applications with autofluorescent backgrounds.
Key Features:
- High Brightness: Conjugates to proteins at high molar ratios without self-quenching, providing brighter signals and enhanced detection.
- Photostability: Resists photobleaching for consistent results.
- Near-Infrared: Long-wavelength emission minimizes background interference in complex samples.
Applications:
- Small Animal Imaging: Excellent clearance profiles in in vivo studies.
- Western Blotting & ELISA: Offers sensitive detection in bioassays.
- Flow Cytometry: Multiparameter detection with minimal autofluorescence.
APDye Fluor 680 is a reliable, cost-effective alternative for sensitive imaging and detection. Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.