2′,7′-Difluorocarboxyfluorescein (also known as DFF) is a fluorescent dye that has been widely used in various research fields, including cell biology, biochemistry, and pharmacology. DFF is a derivative of carboxyfluorescein, a fluorophore that is commonly used for labeling proteins, nucleic acids, and other biomolecules.
The unique properties of DFF, including its high quantum yield and sensitivity to changes in pH, make it an ideal tool for a wide range of applications. DFF can be used as a pH-sensitive probe to monitor changes in pH in live cells, and it can also be used to track the trafficking of proteins and other biomolecules in vivo.
Moreover, DFF has been used in the development of new diagnostic and therapeutic strategies. For example, DFF-based sensors have been used to detect the presence of specific molecules, such as enzymes or neurotransmitters, in biological samples. In addition, DFF has been conjugated with therapeutic agents to target specific cells or tissues, providing a promising approach for the treatment of various diseases.
