BDP R6G is a bright and photostable dye with long-lived fluorescence that is virtually pH-independent. Due to the long fluorescence lifetime, this dye can be used in fluorescence polarization assays and two-photon experiments. The fluorophore has an absorption and emission spectrum similar to that of R6G rhodamine.
BDP R6G
BDP R6G: Spectral Properties and Applications
BDP R6G (Boron-Dipyrromethene Rhodamine 6G) is a bright orange-red fluorescent dye known for its high fluorescence quantum yield and excellent photostability. It is frequently used in molecular biology, cell imaging, and fluorescence-based assays due to its strong signal and distinct spectral properties.
Spectral Properties of BDP R6G:
- Absorption Maximum: ~529 nm
- Emission Maximum: ~545 nm
- Stokes Shift: ~16 nm
- High Quantum Yield: Provides strong and efficient fluorescence, ideal for sensitive detection.
- Photostability: BDP R6G resists photobleaching, maintaining fluorescence intensity even during extended imaging.
- Sharp Spectral Peaks: Narrow absorption and emission peaks minimize spectral overlap, improving signal clarity in multiplexed assays.
Applications of BDP R6G:
- Fluorescence Microscopy: Ideal for visualizing cellular structures and tracking dynamic processes with high precision.
- Flow Cytometry: Used for cell sorting and analysis with clear and distinct signals.
- FRET Studies: Employed in FRET assays to investigate molecular interactions and dynamics with high sensitivity.
- Molecular Probes: Useful in labeling proteins, nucleic acids, and other biomolecules for various biological studies.
BDP R6G combines bright fluorescence, stability, and well-defined spectra, making it a preferred choice for researchers needing reliable and precise fluorescent labeling in diverse experimental setups.