Tetrazine-SS-Biotin CAS:2123482-78-8 is a cutting-edge bioorthogonal reagent designed. It consists of two functional groups, tetrazine and biotin, connected by a disulfide bond. Tetrazine, an electron-deficient diene, is known for its exceptional reactivity in bioorthogonal chemistry, while biotin is a well-established affinity tag, widely used for protein purification and detection.
The tetrazine moiety selectively reacts with strained alkynes, such as cyclopropenes and trans-cyclooctenes, in a process called inverse electron-demand Diels-Alder cycloaddition. This reaction occurs rapidly under physiological conditions and is orthogonal to native biological functionalities, making Tetrazine-SS-Biotin ideal for labeling and tracking biomolecules in live cells.
The biotin component allows for easy affinity-based purification and detection of the labeled biomolecules using streptavidin or avidin-coated surfaces. The disulfide bond provides a cleavable linker that can be reduced to release the biotinylated species, facilitating downstream analysis.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
