Name: DAPI, blue fluorescent nucleic acid stain
SKU: AP15315-1
Availability: InStock

DAPI, blue fluorescent nucleic acid stain

$30.00 – $110.00

DAPI is a blue fluorescent dye that binds AT-rich DNA, enhancing fluorescence for cell imaging. It’s widely used in microscopy, flow cytometry, and as a nuclear stain. With excitation at 358 nm and emission at 461 nm, DAPI stains both fixed and live cells and detects mycoplasma with high sensitivity.

Unit

Price

Quantity

1 mg

$30

10mg

$110

Would you like to inquire about bulk pricing?

DAPI Stain: Key Features and Uses

DAPI (4′,6-diamidino-2-phenylindole), CAS 28718-90-3, is a blue fluorescent dye that binds strongly to AT-rich regions in DNA, enhancing fluorescence by ~20-fold. It’s used in fluorescence microscopy, flow cytometry, and as a nuclear counterstain.

Spectral Data

Excitation: 358 nm
Emission: 461 nm (DNA), shifts to ~500 nm (RNA)

Applications

Microscopy: Stains nuclei in fixed and live cells at high concentrations.
Blotting: Detects annealed probes and DNA in agarose gels.
Mycoplasma Detection: Sensitive, specific staining method.

DAPI Staining Protocol

Prepare Cells: Fix cells with 4% paraformaldehyde for 10 minutes at room temperature and wash with PBS.
Stain with DAPI: Dilute DAPI in PBS to a final concentration of 0.1–1 µg/mL.
Incubate: Add DAPI solution to cells and incubate for 5–10 minutes at room temperature in the dark.
Wash: Rinse cells with PBS to remove excess dye.
Image: Visualize using a fluorescence microscope with DAPI filter settings (excitation 358 nm, emission 461 nm).

Ensure minimal exposure to light to prevent photobleaching.

DAPI’s intense fluorescence and strong DNA binding make it a go-to for cell imaging and chromosome analysis!

AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.

DAPI, blue fluorescent nucleic acid stain

Catalog #:	AP15315-1
Name:	DAPI, blue fluorescent nucleic acid stain
Chemical Formula:	C₁₆H₁₇Cl₂N₅
Molecular Weight:	350.25
CAS:	28718-90-3
Purity:	≥95%
Availability:	In Stock