DAPI
DAPI (4′,6-diamidino-2-phenylindole) is a high-purity blue fluorescent nucleic acid stain that binds especially strongly to A-T rich regions in DNA, making it widely used for nuclear and chromosome staining in fluorescence microscopy and cell imaging.
Key Features and Benefits
-
Bright Blue Fluorescence – Excitation at ~358 nm and emission at ~461 nm for clear nuclear visualization.
-
High DNA Affinity – Selectively binds to double-stranded DNA, especially A-T rich regions.
-
Versatility – Compatible with fixed and live cells (membrane-permeable at higher concentrations).
-
Reliable Performance – High sensitivity with low background fluorescence.
Applications
-
Nuclear and chromosomal staining in fluorescence microscopy
-
Cell cycle and apoptosis studies
-
Counterstain in immunofluorescence assays
-
Flow cytometry and fluorescence in situ hybridization (FISH)
Storage & Handling
-
Storage: Store at –20 °C, protected from light.
-
Handling: Prepare working solutions in aqueous buffer. Protect from repeated freeze–thaw cycles and light exposure.
This product is a widely used blue fluorescent stain that binds DNA with high specificity and brightness, making it especially essential for nuclear imaging, chromosome visualization, and multi-color fluorescence assays.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
