Biotin-PEG-azide is a polyethylene glycol (PEG) derivative that contains an azide functional group (-N_3) and a biotin molecule. The azide group is known for its ability to react with various molecules through copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions, while biotin is a commonly used molecular probe in biochemistry and cell biology research.
Biotin-PEG-azide has found extensive use in the field of pharmaceutical research and development as a versatile tool for the conjugation and labeling of biomolecules. Its unique chemical properties allow for the selective modification of specific molecules, including peptides, proteins, and nucleic acids, with biotin tags, which can be subsequently detected using streptavidin-based assays.
In addition to its use in biomolecule conjugation and labeling, Biotin-PEG-azide has also been employed as a surface modifier in various biomedical applications. For example, it can be immobilized onto the surface of nanoparticles or medical devices to improve their stability, biocompatibility, and targeting ability.
