DBCO-NHCO-PEG-t-butyl ester is a versatile molecule that finds wide applications in drug research and development. It belongs to a class of compounds called click chemistry reagents, which are commonly used to modify biomolecules with high efficiency and specificity. DBCO-NHCO-PEG-t-butyl ester is composed of several functional groups, including an N-hydroxysuccinimide (NHS) ester, a t-butyl ester, and a polyethylene glycol (PEG) spacer. The NHS ester allows for the efficient coupling of the molecule to amine-containing biomolecules, while the PEG spacer imparts water solubility and reduces nonspecific binding. The t-butyl ester can be easily removed under mild conditions, allowing for the controlled release of the attached biomolecules.