APDye Fluor 647: Superior Fluorescent Dye for Precise Labeling
Overview: APDye Fluor 647, an equivalent to Alexa Fluor 647, offers exceptional performance similar to other Cyanine5-based fluorescent dyes like Cy5, Alexa Fluor 647, and CF 647. This bright green-fluorescent dye is ideal for a range of applications, including protein and antibody labeling, as well as nucleic acid assays.
Key Features:
- Spectral Similarity: Nearly identical in spectral properties to Cy5 and Alexa Fluor 647.
- Optimal Excitation: Best used with a 633 or 650 nm Argon laser for effective fluorescence.
- Water Solubility: Highly soluble in water with 4 sulfonate groups, reducing aggregation in aqueous solutions.
- pH Stability: Maintains performance across a wide pH range from 4 to 10.
Benefits:
- High Fluorescence Quantum Yield: Ensures bright, detectable signals for low-abundance biological structures.
- Excellent Photostability: Provides reliable and consistent results with high sensitivity.
- High Labeling Density: Can be attached to proteins and nucleic acids at high molar ratios without significant self-quenching, resulting in brighter conjugates.
Applications:
- Protein and Antibody Labeling: Perfect for high-density labeling in research and diagnostic applications.
- Nucleic Acid Detection: Useful for various nucleic acid applications with high sensitivity.
Product Offerings:
- Conjugated Probes: APDye Fluor 647 conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates, optimized for cellular labeling and detection.
- Reactive Dye Forms and Kits: Available for generating your own antibody conjugates or probes, providing flexibility for custom applications.
Product Citations:
Shoukath Sulthana† a, Abeera Bhatti† b, Elza Mathew ORCID logob, Sohel H. Quazi ac, Natasha N. Gaudreault *b, Robert DeLong ORCID logo*d and Santosh Aryal; Synthetic graphene–copper nanocomposites interact with the hACE-2 enzyme and inhibit its biochemical activity; Nanoscale Adv., 2024, 6, 188-196
