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APDye Fluor 555 (AF555, Alexa Fluor 555 equivalent) is a water-soluble, bright orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. APDye Fluor 555 has maximum excitation and emission wavelengths of 555/565 nm and can be excited efficiently using the 543 nm He-Ne laser line. APDye Fluor 555 conjugates of antibodies, peptides, and proteins are pH insensitive from pH 4 to pH 10. APDye Fluor 555 molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

APDye Fluor 555 is superior to Cy3 alternatives, APDye Fluor 555 matches the spectrum of Cy3 conjugates and is therefore suitable for Cy3 filters. APDye Fluor 555 has higher total fluorescence and better photostability, providing researchers with longer image capture times.

APDye Fluor 555 antibody conjugates are suitable for a variety of applications, including flow cytometry, fluorescence microscopy, immunohistochemical techniques, primary detection, ELISA, immunocytochemical techniques, indirect FISH, and more.

Cat# Name Structure M.W. Purity Pricing
AP12433APDye 555 Alkyne884.06≥95% Pricing
AP12639CalFluor 555 Azide891.09≥95% Pricing
AP12431APDye 555 Azide928.25 (protonated)≥95% Pricing
AP12429APDye 555 NHS ester944.07 (protonated)≥95% Pricing
AP14425AP555 carboxylic acid961.29≥95% Pricing
AP14038APDye 555 Tyramide966.16 (protonated)≥95% Pricing
AP12430APDye 555 Maleimide969.12≥95% Pricing
AP12432APDye 555 Picolyl Azide1049.21≥95% Pricing
AP12667APDye 555 Azide Plus1067.28 (protonated)≥95% Pricing
AP12434APDye 555 DBCO1105.32≥95% Pricing

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