TAMRA Biotin
TAMRA Biotin: Overview, Features, and Applications
TAMRA Biotin combines the red-orange fluorescent dye TAMRA (Carboxytetramethylrhodamine) with biotin, creating a powerful tool for molecular biology and biochemical research. This conjugate is widely used for labeling biomolecules, allowing for simultaneous fluorescent detection and biotin-avidin/streptavidin binding, enhancing the versatility of fluorescence-based assays.
Key Features of TAMRA Biotin:
- Fluorescence Properties:
- Absorption Maximum: ~555 nm
- Emission Maximum: ~580 nm
- Bright and Stable Fluorescence: Offers high-intensity red-orange fluorescence with excellent photostability.
- Biotin Functionality: Allows for strong, specific binding to avidin or streptavidin, enabling efficient capture, purification, or detection of labeled molecules.
- Dual Functionality: Combines the strong fluorescent signal of TAMRA with the specific binding properties of biotin, making it ideal for dual-detection applications.
Applications of TAMRA Biotin:
- Fluorescence Microscopy: Enables the visualization of biotinylated targets with bright red-orange fluorescence, suitable for cellular imaging and tracking.
- Protein and Nucleic Acid Labeling: Commonly used for labeling proteins, peptides, and nucleic acids for detection in fluorescence-based assays.
- FRET Assays: TAMRA acts as a donor or acceptor in FRET, providing insights into molecular interactions when combined with biotin’s binding properties.
- Biotin-Avidin/streptavidin Binding Assays: Facilitates capture, enrichment, or detection of labeled molecules, enhancing assay sensitivity and specificity.
TAMRA Biotin’s combination of bright fluorescence and biotin’s binding capabilities makes it a valuable tool for advanced research in molecular biology, diagnostics, and biochemical assays.