Sulfo-Cyanine5.5 (Sulfo-Cy5.5) is a far-red, water-soluble fluorescent dye with an absorption maximum at ~675 nm and emission maximum at ~694 nm. Known for its high photostability and bright NIR fluorescence, it’s perfect for labeling proteins, nucleic acids, and biomolecules, making it ideal for fluorescence microscopy, flow cytometry, in vivo imaging, and bioconjugation in aqueous environments.
sulfo-Cyanine5.5
Sulfo-Cyanine5.5 Dye: Spectral Properties and Applications
Sulfo-Cyanine5.5 (Sulfo-Cy5.5) is a far-red, water-soluble fluorescent dye widely used in biological and biomedical research. As a member of the cyanine dye family, it offers excellent photostability, high quantum yield, and strong NIR fluorescence. Sulfo-Cy5.5’s sulfonate groups enhance its solubility in water, making it ideal for labeling proteins, nucleic acids, and other biomolecules in aqueous environments.
Spectral Properties of Sulfo-Cyanine5.5 Dye:
- Absorption Maximum: ~675 nm
- Emission Maximum: ~694 nm
- Stokes Shift: ~19 nm
- High Quantum Yield: Produces bright and efficient far-red fluorescence.
- Enhanced Water Solubility: Sulfonate groups improve performance in biological assays.
Applications of Sulfo-Cyanine5.5 Dye:
- Fluorescence Microscopy: Provides high-contrast imaging of cellular structures.
- Flow Cytometry: Offers distinct signals for accurate cell sorting and analysis.
- In Vivo Imaging: Ideal for deep tissue imaging with minimal background noise.
- Bioconjugation: Effectively labels proteins, antibodies, and nucleic acids.
- Molecular Probes: Used in bioanalytical assays to study molecular interactions.
Sulfo-Cy5.5’s bright fluorescence, high stability, and enhanced solubility make it a valuable tool for researchers needing reliable labeling in complex biological and biomedical applications.