Cyanine5 Dye
Cyanine 5 Dye (Cy5)
Overview: Cyanine 5 (Cy5) is a fluorescent dye renowned for its bright and stable fluorescence. It is widely used in various scientific applications, including imaging and molecular biology.
Key Features:
Fluorescence Properties:
Absorption Maximum: ~649 nm
Emission Maximum: ~670 nm
Quantum Yield: High, around 0.28
Brightness: Cy5 is highly fluorescent, making it easy to detect even at low concentrations.
Stability: It is very stable and resists fading (photobleaching) during extended use.
Applications:
Molecular Imaging: Ideal for fluorescence microscopy to visualize biological samples.
Flow Cytometry: Used for analyzing cells and particles in flow cytometers.
Western Blotting: Helps in detecting and quantifying proteins on blots.
Fluorescent Probes: Serves as a tag in various assays, including ELISA and FRET.
Benefits:
High Sensitivity: Produces strong signals for precise detection and quantification.
Versatile Use: Suitable for a range of research and clinical applications.
Durable: Maintains performance over time, reducing the need for frequent re-testing.
Summary: Cyanine 5 Dye (Cy5) is a versatile and reliable fluorescent dye, offering strong and stable fluorescence for enhanced imaging and detection in scientific research.
Ref:
Ma, X., Shi, L., Zhang, B. et al. Recent advances in bioprobes and biolabels based on cyanine dyes. Anal Bioanal Chem 414, 4551–4573 (2022).