BDP 630/650 is a bright red fluorescent dye with absorption around 630 nm and emission at 650 nm, offering high photostability and a sharp emission profile. It is ideal for fluorescence imaging, FRET, and flow cytometry, providing clear signals with minimal spectral overlap, making it perfect for advanced biological and chemical research applications.
BDP 630/650 has excellent photostability to cyanine. It can replace Cyanine5 and sulfo-Cyanine5 for longer excited state lifetime. This makes it beneficial for applications such as fluorescence polarization assays.
BDP 630/650
BDP 630/650: Spectral Properties and Applications
BDP 630/650 (Boron-Dipyrromethene 630/650) is a red fluorescent dye known for its excellent photostability, high quantum yield, and well-defined spectral properties. It is widely used in fluorescence imaging, FRET, and flow cytometry, providing bright and reliable signals in various biological and chemical research applications.
Spectral Properties of BDP 630/650:
- Absorption Maximum: ~630 nm
- Emission Maximum: ~650 nm
- Stokes Shift: ~20 nm
- High Quantum Yield: Offers strong and efficient fluorescence, ideal for sensitive detection.
- Photostability: Resists photobleaching, maintaining consistent fluorescence during prolonged experiments.
- Sharp Spectral Peaks: Narrow absorption and emission spectra reduce overlap, enhancing signal clarity in multiplex assays.
Applications of BDP 630/650:
- Fluorescence Microscopy: Ideal for high-resolution imaging of cellular structures and dynamic processes.
- Flow Cytometry: Provides distinct, bright signals for cell sorting and phenotyping.
- FRET Studies: Used in FRET assays to study molecular interactions and dynamics with high sensitivity.
- Molecular Labeling: Effective for labeling proteins, nucleic acids, and other biomolecules.
BDP 630/650’s bright red fluorescence, stability, and precise spectral properties make it a reliable choice for researchers seeking high-performance fluorescent dyes for advanced studies.