DBCO PEG NHS linkers have popular copper free click chemistry DBCO moiety on one end and an active NHS ester group on the other.
The NHS ester functional group is widely used to conjugate the crosslinker with biomolecules with amino functional group. The resulting biomolecule bearing DBCO functional group can undergo a click chemistry with biomolecules bearing azide group. The process has no toxic metal catalyst involved and is fast and high yielding
PEG spacers add enhanced aqueous solubility and make the crosslinkers suitable for in vivo bioconjugation applications.