SPDP-PEG4-Acid (CAS: 581065-97-6)
SPDP-PEG4-Acid combines a cleavable SPDP (N-succinimidyl 3-(2-pyridyldithio)propionate) crosslinker with a terminal carboxylic acid group, making it a versatile tool for bioconjugation and molecular research. The SPDP moiety reacts with thiol groups, forming reversible disulfide bonds, while the carboxylic acid readily couples with primary amines when activated by reagents such as EDC or HATU. The PEG4 spacer increases water solubility and provides flexibility, reducing steric hindrance in complex conjugation reactions.
Key Features:
- Reactive Groups:
- SPDP Crosslinker: Forms cleavable disulfide bonds with thiol groups, enabling reversible conjugation.
- Carboxylic Acid: Couples with primary amines after activation, forming stable amide bonds.
- PEG4 Spacer:
- Enhances solubility in aqueous environments.
- Increases molecular flexibility, reducing steric hindrance during coupling reactions.
Applications:
- Bioconjugation:
- Links biomolecules such as proteins and peptides through thiol and amine-reactive chemistry, aiding in targeted drug development and diagnostics.
- PROTAC Synthesis:
- Functions as a linker for proteolysis-targeting chimeras (PROTACs), facilitating targeted protein degradation through the ubiquitin-proteasome system.
Handling and Storage:
- Molecular Formula: C₁₉H₃₀N₂O₇S₂
- Molecular Weight: 462.58 g/mol
- Storage Recommendations:
- Store at -20°C in a cool, dry place, away from light and moisture, to ensure long-term stability.
SPDP-PEG4-Acid excels in advanced molecular research due to its dual-reactive design and water-soluble PEG4 spacer. Its ability to form both stable and reversible bonds makes it essential for chemical biology, pharmaceutical development, and targeted therapeutic design.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
