Fmoc-Lys(biotin-PEG4)-OH
This product is an Fmoc-protected lysine derivative functionalized with a biotin tag via a PEG4 spacer. The Fmoc group protects the α-amino group for solid-phase peptide synthesis (SPPS), while the ε-amino side chain is modified with biotin-PEG4, enabling biotin incorporation into peptides. The PEG4 spacer specifically improves solubility and accessibility, ensuring efficient biotin–streptavidin binding in peptide conjugates.
Key Features and Benefits
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Fmoc Protection: Compatible with standard SPPS workflows, removable under mild basic conditions.
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Biotin Tag: Provides strong, specific binding to streptavidin, avidin, and NeutrAvidin systems.
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PEG4 Spacer: Enhances solubility, flexibility, and biotin accessibility in peptide structures.
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Peptide-Compatible Design: Tailored for direct incorporation into synthetic peptides.
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High Purity: Produced to ≥95% purity for reliable peptide synthesis.
Applications
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Peptide Synthesis: Incorporates biotin into peptides during SPPS for downstream assays.
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Affinity Capture: Enables immobilization of biotinylated peptides on streptavidin-coated surfaces.
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Probe Development: Builds biotin-tagged peptides for imaging, diagnostics, and pull-down assays.
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Chemical Biology: Facilitates peptide-based labeling and affinity enrichment.
Storage and Handling
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Storage: Store at –20 °C in a sealed, desiccated container.
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Handling: Use under dry conditions; avoid repeated freeze–thaw cycles.
This product provides a convenient way to introduce biotin into peptides with improved solubility and binding performance, making it especially useful for biochemical and diagnostic applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.