FAM (5,6)-isothiocyanate, or (5,6)-FITC (CAS 27072-45-3), is an isothiocyanate derivative of fluorescein that reacts with nucleophilic groups such as amino, hydroxyl, and thiol on biomolecules. It is widely used to label antibodies and other probes for various fluorescence-based techniques, including microscopy, flow cytometry, immunofluorescence, immunochemistry, Western blotting, and ELISA.
Key Features:
- Molecular Formula: C21H11NO5S
- Molecular Weight: 389.39 g/mol
- Fluorescence: Excitation at 494 nm, emission at 518 nm.
- Applications: Labeling biomolecules for detection in fluorescence assays.
Handling and Storage:
- Store in a cool, dark place to protect from light and moisture.
- Use gloves and safety glasses when handling to avoid contact.
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