DiD (1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindodicarbocyanine Perchlorate): Comprehensive Guide
Overview
DiD (CAS 75539-51-4) is a far-red fluorescent carbocyanine dye used to label cell membranes in vivo and in vitro. It integrates into lipid bilayers, staining cells with bright fluorescence ideal for tracking neuronal activity in anterograde and retrograde directions. DiD remains confined to labeled cells in intact tissues, with minimal transfer unless membrane integrity is compromised.
Key Features
- Membrane Labeling: Stains entire cells by lateral diffusion in membranes.
- Applications: Suitable for cell tracking, neural tracing, and dual-color studies with DiI and DiO.
- Solid Form Use: Direct application of dye crystals onto cell membranes.
Benefits
- Bright, Stable Fluorescence: Enhances visualization of cell dynamics.
- Minimal Crosstalk: Maintains clear labeling in healthy tissues.
DiD’s robust and stable fluorescence makes it a valuable tool in neurobiology, cell biology, and tissue imaging studies.
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