Diazo Biotin-PEG3-alkyne

Diazo-Biotin-PEG3-alkyne

This product is a multifunctional linker combining a diazo group, a biotin tag, a PEG3 spacer, and a terminal alkyne. The diazo group enables photoaffinity labeling through covalent insertion into C–H or heteroatom–H bonds, while the alkyne allows click chemistry with azides. The biotin provides strong affinity binding to streptavidin or avidin systems, and the PEG3 spacer specifically improves solubility and reduces steric hindrance.

Key Features and Benefits

• Diazo Group: Enables photoaffinity labeling for covalent capture of binding partners.

• Biotin Tag: Strong, specific binding to streptavidin and avidin systems.

• Terminal Alkyne: Supports CuAAC click chemistry with azides.

• PEG3 Spacer: Adds flexibility, solubility, and accessibility.

• High Purity: Produced to ≥95% purity for reliable experimental use.

Applications

• Photoaffinity Labeling: Captures transient biomolecular interactions under UV activation.

• Bioconjugation: Couples biomolecules through alkyne–azide click chemistry.

• Affinity Capture: Enriches targets using biotin–streptavidin binding.

• Probe Development: Builds multifunctional probes for imaging, proteomics, and target identification.

Storage and Handling

• Storage: Store at –20 °C in a sealed, light- and moisture-protected container.

• Handling: Protect from light; prepare fresh solutions before use.

This product integrates photo-crosslinking, click chemistry, and biotin affinity capture in a compact PEG scaffold, specifically making it a powerful tool for proteomics and chemical biology research.

AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.