DBCO-PEG5-NHS ester
This product is a heterobifunctional linker containing a dibenzocyclooctyne (DBCO) group, a PEG5 spacer, and an NHS ester. The DBCO enables rapid, copper-free SPAAC click chemistry with azides, while the NHS ester couples efficiently with primary amines to form stable amide bonds. The PEG5 spacer improves solubility and flexibility, making this reagent especially useful for protein labeling, probe design, and bioconjugation.
Key Features and Benefits
-
DBCO Group: Supports ultrafast, copper-free click ligation with azides.
-
NHS Ester: Reacts efficiently with primary amines (lysines, N-termini).
-
PEG5 Spacer: Provides solubility, flexibility, and reduced steric hindrance.
-
Dual Reactivity: Combines amine coupling with click chemistry.
-
High Purity: Produced to ≥95% purity for reproducible applications.
Applications
-
Protein and Antibody Labeling: Couples NHS ester to amines for site-specific modification.
-
Bioconjugation: Links azide- and amine-functionalized biomolecules.
-
Probe Development: Builds dual-reactive imaging and diagnostic probes.
-
Drug Delivery: Serves as a linker for targeted therapeutic systems.
Storage and Handling
-
Storage: Store at –20 °C in a sealed, desiccated container.
-
Handling: Prepare fresh solutions; avoid prolonged exposure to aqueous buffers.
This product integrates copper-free DBCO click reactivity with amine-coupling NHS chemistry, providing an especially versatile tool for efficient bioconjugation and probe development.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
