Cyanine3B DBCO is a yellow-emitting cyanine dye designed for efficient bioorthogonal labeling. It uses the highly reactive DBCO (dibenzocyclooctyne) group, which enables fast copper-free click chemistry (SPAAC) with azides. Unlike other cyclooctynes, DBCO doesn’t react with tetrazines, making it ideal for use with trans-cyclooctenes and tetrazines in bioorthogonal reactions.
Cyanine3B stands out for its higher fluorescence quantum yield and photostability compared to Cyanine3, thanks to its fixed conformation. Plus, its sulfonated structure allows for effective labeling in water media, making it perfect for live cell imaging.
Key Features:
- Reactivity: DBCO group for rapid azide reactions
- Fluorescence: Bright yellow with high quantum yield
- Photostability: Superior to Cyanine3
- Compatibility: Works with trans-cyclooctenes and tetrazines
- Cyanine3B DBCO Applications:
- Biomolecule labeling in aqueous environments
- Live cell imaging
- Fluorescence microscopy
- Flow cytometry
With these properties, Cyanine3B DBCO offers an excellent choice for fluorescence studies in biological systems.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
