Biotin-PEG11-NH-Mal
Biotin-PEG11-NH-Mal is a high-purity heterobifunctional PEG linker featuring a biotin group for strong avidin/streptavidin binding and a maleimide group for thiol-specific conjugation. The PEG11 spacer provides excellent aqueous solubility, high flexibility, and reduced steric hindrance, enabling efficient bioconjugation in both aqueous and organic systems. This design specifically allows site-specific attachment of biotin to thiol-containing biomolecules, nanoparticles, or surfaces for use in detection, purification, and targeted delivery applications.
Key Features and Benefits
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Biotin Moiety: Enables high-affinity binding to avidin, streptavidin, or neutravidin systems.
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Maleimide Group: Selectively reacts with thiols to form stable thioether bonds under mild conditions.
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PEG11 Spacer: Enhances solubility, flexibility, and accessibility of reactive groups.
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Heterobifunctional Design: Supports orthogonal conjugation workflows for multifunctional constructs.
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High Purity: Supplied at >95% purity for reproducible, reliable performance.
Applications
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Protein and Peptide Labeling: Attach biotin to thiol-containing biomolecules for affinity-based detection or capture.
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Affinity Purification: Facilitate immobilization of thiol-modified ligands to streptavidin-coated surfaces.
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Nanoparticle Functionalization: Add biotin for targeted binding and delivery.
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Diagnostic Probe Development: Create biotinylated imaging and sensing agents.
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Biosensor Fabrication: Immobilize biomolecules for high-sensitivity detection systems.
Storage and Handling
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Storage: Store at –20 °C, dry, and protected from light.
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Handling: Use anhydrous solvents such as DMF or DMSO; avoid moisture to preserve maleimide reactivity.
Biotin-PEG11-NH-Mal combines strong biotin–streptavidin binding with thiol-specific maleimide chemistry in a long, hydrophilic PEG11 spacer, making it especially ideal for bioconjugation in detection, purification, and targeted delivery applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
