3-(2-Iodoethyl)-3-methyl-3H-diazirine CAS:1002754-71-3 is a small molecule that is used in chemical biology and biochemistry research as a photo-crosslinking agent. It contains several functional groups, including a diazirine group, an iodine atom, and an ethyl and methyl group.
The diazirine group is the key functional group in 3-(2-Iodoethyl)-3-methyl-3H-diazirine, and it is responsible for its photo-crosslinking properties. When exposed to ultraviolet light, the diazirine group undergoes a reaction that forms a highly reactive carbene intermediate. This intermediate can then react with nearby molecules, including proteins and nucleic acids, leading to the formation of covalent bonds and the crosslinking of these molecules.
The iodine atom in 3-(2-Iodoethyl)-3-methyl-3H-diazirine is used as a radiolabel, allowing researchers to track the molecule and its interactions with other molecules in a biological system. The ethyl and methyl groups are important for the chemical properties and stability of the compound.
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