TCO-PEG6-maleimide
This product is a heterobifunctional linker featuring a trans-cyclooctene (TCO) group, a PEG6 spacer, and a maleimide functionality. The TCO enables rapid copper-free click chemistry with tetrazines, while the maleimide group reacts selectively with thiols to form stable thioether bonds. The PEG6 spacer specifically improves solubility and flexibility, reducing steric hindrance during conjugation.
Key Features and Benefits
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TCO Group: Provides ultrafast, copper-free click reactivity with tetrazines via inverse electron-demand Diels–Alder (IEDDA) chemistry.
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Maleimide Group: Reacts selectively with thiols on cysteine residues to form stable linkages.
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PEG6 Spacer: Enhances aqueous solubility, reduces steric hindrance, and provides conformational flexibility.
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Dual Reactivity: Combines bioorthogonal click chemistry with thiol-specific conjugation.
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High Purity: Manufactured to ≥95% purity for consistent and reproducible performance.
Applications
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Bioconjugation: Couples thiol-containing biomolecules with tetrazine-functionalized partners.
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Antibody and Protein Labeling: Enables site-specific conjugation through cysteine residues.
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Drug Delivery Research: Serves as a linker for targeted therapeutics and ADC development.
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Chemical Biology: Facilitates orthogonal labeling strategies in complex biological systems.
Storage and Handling
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Storage: Store at –20 °C in a tightly sealed container, protected from light and moisture.
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Handling: Avoid repeated freeze–thaw cycles; dissolve freshly before use in dry solvent.
This product integrates TCO click chemistry with thiol-reactive maleimide functionality, making it an especially versatile linker for bioconjugation, protein labeling, and drug delivery applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
