TCO-NHS ester
This product is a heterobifunctional reagent containing a trans-cyclooctene (TCO) group and an N-hydroxysuccinimide (NHS) ester. The TCO enables ultrafast copper-free click chemistry with tetrazines, while the NHS ester reacts efficiently with primary amines to form stable amide bonds. This combination makes it an especially powerful tool for site-specific bioconjugation and probe development.
Key Features and Benefits
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TCO Group: Provides rapid, copper-free ligation with tetrazines via inverse electron-demand Diels–Alder (IEDDA) chemistry.
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NHS Ester: Couples selectively with primary amines on proteins, peptides, and surfaces.
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Dual Reactivity: Combines bioorthogonal click chemistry with amine-targeted conjugation.
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High Efficiency: Supports fast, selective reactions under mild conditions.
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High Purity: Produced to ≥95% purity for reproducible results.
Applications
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Bioconjugation: Links TCO-tagged molecules to amine-containing biomolecules.
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Protein and Antibody Labeling: Enables site-specific modification via lysine residues or N-terminal amines.
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Probe Development: Builds dual-functional probes for imaging and diagnostics.
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Drug Delivery Research: Supports development of targeted therapeutics using orthogonal chemistry.
Storage and Handling
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Storage: Store at –20 °C in a sealed, moisture- and light-protected container.
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Handling: Dissolve freshly in dry solvent; avoid repeated freeze–thaw cycles.
This product integrates fast TCO click reactivity with efficient NHS ester conjugation, offering an especially versatile platform for protein labeling, probe development, and advanced bioconjugation.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
