SMPH Crosslinker
This product is a heterobifunctional crosslinker containing an NHS ester and a maleimide group separated by a 9.5 Å spacer arm. The NHS ester reacts with primary amines, while the maleimide couples selectively with sulfhydryls, enabling stable amide and thioether bond formation. Its medium-length spacer specifically provides flexibility for efficient bioconjugation and protein–protein crosslinking.
Key Features and Benefits:
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NHS Ester: Rapidly reacts with primary amines on proteins, peptides, or surfaces.
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Maleimide Group: Selectively couples with thiols to form stable thioether linkages.
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Spacer Length (~9.5 Å): Provides optimal distance for reducing steric hindrance.
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High Purity: Ensures reproducibility in bioconjugation and crosslinking workflows.
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Versatile Use: Widely applied in protein conjugation, antibody labeling, and assay development.
Applications:
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Protein Crosslinking: Links amine- and thiol-containing biomolecules with precision.
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Antibody Labeling: Facilitates site-specific conjugation for assay and therapeutic use.
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Bioconjugation: Creates stable amide and thioether linkages for robust conjugates.
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Surface Functionalization: Functionalizes biomaterials, nanoparticles, and biosensors.
Storage and Handling:
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Storage: Store desiccated at –20 °C, protected from light and moisture.
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Handling: Prepare fresh stock solutions in anhydrous solvents; avoid repeated freeze–thaw cycles.
This product provides efficient thiol-to-amine conjugation with a medium-length spacer, making it an especially reliable tool for bioconjugation, labeling, and assay development.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
