N-(5-Aminopentyl)maleimide Trifluoroacetate
N-(5-Aminopentyl)maleimide trifluoroacetate is a high-purity heterobifunctional crosslinker featuring a maleimide group for site-specific conjugation to thiols and a primary amine for versatile coupling with activated esters or other electrophiles. The 5-carbon alkyl spacer provides flexibility and optimal spacing between functional groups, while the trifluoroacetate (TFA) salt form improves handling and stability. This reagent is especially ideal for protein, peptide, and nanoparticle modification where controlled, orthogonal bioconjugation is required.
Key Features and Benefits
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Maleimide Group – Selectively reacts with sulfhydryl groups via Michael addition under mild conditions.
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Primary Amine – Compatible with NHS esters, isocyanates, and activated acids for versatile conjugation.
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5-Carbon Spacer – Enhances molecular flexibility and reduces steric hindrance between reactive sites.
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TFA Salt Form – Improves compound stability and ease of handling in solid form.
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High Purity – Supplied at >95% purity for reproducible performance in sensitive bioconjugation applications.
Applications
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Protein and peptide thiol-specific labeling
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Crosslinking for antibody–drug conjugates (ADCs)
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Surface modification of nanoparticles and biomaterials
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Dual-functional molecule synthesis requiring orthogonal reactivity
Storage and Handling
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Storage: Store at –20 °C, dry, and protected from light
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Handling: Use anhydrous solvents such as DMSO or DMF; avoid prolonged exposure to moisture to preserve maleimide reactivity
This product is a high-purity heterobifunctional reagent with maleimide–thiol specificity and primary amine versatility, linked by a flexible pentyl chain and stabilized as a TFA salt specifically for reliable orthogonal bioconjugation.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
