Methyltetrazine-Maleimide (Methyltetrazine-Mal) is a bifunctional linker designed for site-specific bioconjugation. It features a tetrazine group for bioorthogonal click chemistry and a maleimide group for thiol-specific conjugation, enabling dual functionality in labeling and crosslinking.
Key Features
- Bioorthogonal Methyltetrazine
Reacts selectively and rapidly with strained alkenes (e.g., trans-cyclooctene) via inverse electron demand Diels-Alder (IEDDA) reactions. - Thiol-Specific Maleimide
Forms stable thioether bonds with free thiol groups in proteins, peptides, or other molecules. - Dual Functionalities
Allows sequential or orthogonal conjugation, enhancing versatility for complex bioconjugation tasks.
Methyltetrazine-Maleimide Applications
- Protein and Antibody Labeling
Enables precise attachment of fluorophores, drugs, or other molecules. - Crosslinking Studies
Facilitates the creation of multifunctional constructs and probes. - Drug Delivery Systems
Useful in designing targeted therapeutic conjugates. - Material Functionalization
Supports modification of surfaces, nanoparticles, and hydrogels.
Advantages
This linker combines bioorthogonal and thiol-reactive chemistries, offering precision and efficiency in diverse applications. It is widely used in bioconjugation, imaging, and targeted delivery research.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
