Mal-PEG4-NHS Ester
Mal-PEG4-NHS ester is a high-purity, heterobifunctional crosslinker containing a maleimide group for thiol-specific conjugation and an NHS ester for amine-reactive coupling. The PEG4 spacer specifically enhances solubility, reduces steric hindrance, and provides optimal spacing between functional groups for efficient bioconjugation. This reagent enables stable thioether bond formation with sulfhydryl-containing molecules and amide bond formation with primary amines, making it ideal for creating well-defined biomolecule conjugates.
Key Features and Benefits:
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Dual Reactivity: Maleimide for sulfhydryl-specific coupling; NHS ester for amine conjugation.
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PEG4 Spacer: Improves aqueous solubility and flexibility, ensuring efficient coupling.
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Stable Linkage: Forms irreversible thioether and amide bonds for long-term conjugate stability.
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High Purity: Supplied at >95% purity for reliable and reproducible results.
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Versatile Use: Compatible with both aqueous and organic synthesis environments.
Applications:
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Protein–Protein Crosslinking: Join proteins via thiol and amine groups.
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Antibody–Drug Conjugates (ADCs): Link cytotoxic drugs to antibodies with precision.
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Surface Functionalization: Modify nanoparticles, hydrogels, or biosensors.
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Enzyme–Substrate Labeling: Create stable enzyme conjugates for assays.
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Targeted Delivery Systems: Functionalize carriers for specific cell targeting.
Storage and Handling:
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Storage: Store at –20 °C in a dry, light-protected environment.
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Handling: Use anhydrous solvents (e.g., DMSO, DMF) and avoid moisture to preserve reactivity.
Mal-PEG4-NHS ester is a especially versatile heterobifunctional PEG linker that enables precise thiol and amine coupling through maleimide and NHS ester chemistry. Its PEG4 spacer improves solubility and flexibility, making it an essential reagent for bioconjugation, ADC synthesis, and targeted delivery systems.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
