(E,E)-3,5-Bis(phenylethenyl)-BDP: Advanced Fluorescent Probe for Lipid Droplet Imaging
What is (E,E)-3,5-Bis(phenylethenyl)-BDP?
(E,E)-3,5-Bis(phenylethenyl)-BDP is a specialized dye used for fluorescence imaging. It belongs to the boron difluoride dipyrromethene (BDP) family and is designed to effectively target and visualize lipid droplets (LDs) in biological samples.
Key Features
- Symmetric Design: This dye features two phenylethenyl groups arranged in a symmetric, trans configuration. This design enhances the molecule’s ability to interact with lipids.
- Extended π-Conjugation: The dye’s aromatic and alkenyl substitutions at the 3,5-positions extend its π-conjugation. This results in a red-shifted fluorescence emission, offering clearer and more distinct imaging compared to traditional BDP dyes.
Why Use (E,E)-3,5-Bis(phenylethenyl)-BDP?
- Bright Fluorescence: It provides strong, clear fluorescence, making it ideal for detecting lipid droplets with high precision.
- Stability: The dye is highly stable under light, ensuring consistent results during extended imaging sessions.
- Lipophilic Nature: Its design allows it to accumulate efficiently in lipid droplets, improving the contrast and visibility of these structures in your samples.
Applications
- Lipid Droplet Imaging: Perfect for visualizing lipid droplets within cells and tissues. It offers enhanced contrast and detailed images.
- Fluorescence Microscopy: Its unique fluorescence properties make it suitable for various microscopy techniques, providing clear and accurate results.
Conclusion
(E,E)-3,5-Bis(phenylethenyl)-BDP is an advanced fluorescent probe that offers superior brightness, stability, and lipophilicity. It’s an excellent choice for researchers needing detailed imaging of lipid droplets.
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