DiR (1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide, CAS: 100068-60-8) is a near-infrared (NIR) fluorescent lipophilic tracer used for labeling cell membranes and hydrophobic structures in both in vivo and in vitro studies.
Key Features:
- Near-Infrared Fluorescence: Excitation at ~748 nm and emission at ~780 nm, ideal for deep tissue imaging.
- Lipophilic Nature: Long alkyl chains integrate into lipid bilayers for effective membrane labeling.
- Stable Membrane Incorporation: Once in membranes, DiR shows minimal transfer between cells.
Applications:
- Cell Membrane Labeling: Stains cell membranes for fluorescence microscopy and flow cytometry.
- In Vivo Imaging: Enables deep tissue imaging in live animals, tracking cell migration and biodistribution.
- Neuronal Tracing: Maps neural pathways using anterograde and retrograde tracing.
Handling and Storage:
- Solubility: Dissolves in organic solvents like DMSO and ethanol.
- Storage: Store at -20°C, protected from light, to ensure long-term stability.
DiR’s strong NIR fluorescence and stable membrane integration make it an essential tool for biomedical research, cell tracking, and in vivo imaging studies.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
