Diazo-Biotin-PEG3-azide
This product is a multifunctional linker combining a diazo group, a biotin tag, a PEG3 spacer, and a terminal azide. The diazo group enables photoaffinity labeling by covalently inserting into C–H or heteroatom–H bonds under UV activation, while the azide allows copper-catalyzed or copper-free click chemistry with alkynes. The biotin specifically ensures strong affinity capture, and the PEG3 spacer improves solubility and flexibility.
Key Features and Benefits
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Diazo Group: Enables photo-crosslinking for covalent capture of transient interactions.
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Biotin Tag: Provides high-affinity binding to streptavidin, avidin, or NeutrAvidin.
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Azide Group: Reacts efficiently with alkynes via CuAAC or SPAAC click chemistry.
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PEG3 Spacer: Enhances solubility and minimizes steric hindrance.
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High Purity: Manufactured to ≥95% purity for reproducible applications.
Applications
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Photoaffinity Labeling: Captures dynamic biomolecular interactions under UV activation.
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Bioconjugation: Couples biomolecules through azide–alkyne click reactions.
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Affinity Capture: Enriches conjugates using biotin–streptavidin systems.
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Probe Development: Builds multifunctional probes for proteomics, imaging, and diagnostics.
Storage and Handling
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Storage: Store at –20 °C in a sealed, desiccated container, protected from light.
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Handling: Use freshly prepared solutions; avoid repeated freeze–thaw cycles.
This product integrates photo-crosslinking, click chemistry, and biotin affinity capture in a compact PEG3 design, making it an especially powerful reagent for proteomics and chemical biology.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
