CalFluor 647 Azide
This product is a far-red fluorescent dye containing an azide group. It specifically enables copper-catalyzed or copper-free click chemistry with alkynes, providing bright and photostable fluorescence for labeling, imaging, and probe development.
Key Features and Benefits
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Azide Group: Reacts efficiently with alkynes via CuAAC or SPAAC click chemistry.
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Far-Red Fluorescence: Emits strong signal (Ex/Em ≈ 646/664 nm).
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Photostability: Provides reliable performance in long-term imaging experiments.
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High Sensitivity: Detects low-abundance targets in microscopy, flow cytometry, and assays.
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High Purity: Ensures reproducibility in sensitive labeling workflows.
Applications
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Bioconjugation: Labels proteins, peptides, and nucleic acids through click chemistry.
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Probe Development: Builds fluorescent probes for diagnostics and research.
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Cell Imaging: Provides bright far-red signal for live or fixed cell studies.
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Assay Development: Useful in FRET, ELISA, and fluorescence-based detection.
Storage and Handling
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Storage: Store at –20 °C in a sealed, light-protected container.
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Handling: Dissolve in DMSO or aqueous buffers; avoid repeated freeze–thaw cycles.
This product integrates azide click reactivity with far-red fluorescence, specifically offering a versatile dye for bioconjugation, imaging, and probe development.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
