Bis-PEG25-NHS ester
Bis-PEG25-NHS ester is a high-purity homobifunctional PEG crosslinker containing two NHS ester groups for efficient amine-to-amine conjugation. The long PEG25 spacer significantly improves aqueous solubility, provides high flexibility, and minimizes steric hindrance, enabling crosslinking of large biomolecules or surfaces in both aqueous and organic environments. With its extended hydrophilic spacer, this reagent is especially ideal for applications requiring increased spacing, reduced aggregation, and enhanced biocompatibility.
Key Features and Benefits
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Dual NHS Ester Groups: Efficiently react with primary amines to form stable amide bonds.
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Long PEG25 Spacer: Provides extended distance between conjugated molecules to minimize steric effects.
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High Water Solubility: Facilitates reactions in physiological and high-salt buffers.
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Homobifunctional Design: Ideal for symmetric crosslinking of proteins, peptides, and surfaces.
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High Purity: Supplied at >95% purity for reproducibility and reliable performance.
Applications
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Protein–Protein Crosslinking: Couple antibodies, enzymes, or other amine-containing biomolecules.
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Surface Functionalization: Modify nanoparticles, hydrogels, or biosensors with amine-reactive handles.
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PEGylation: Introduce long PEG chains to improve pharmacokinetics and reduce immunogenicity.
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Drug Delivery Systems: Build stable conjugates for enhanced stability and circulation time.
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Biomaterials Engineering: Create hydrophilic, flexible linkages in polymeric or hybrid materials.
Storage and Handling
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Storage: Store at –20 °C, dry, and protected from light.
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Handling: Use anhydrous solvents such as DMF or DMSO; avoid moisture to preserve NHS ester reactivity.
Bis-PEG25-NHS ester offers dual amine-reactive functionality with an exceptionally long, hydrophilic PEG spacer, making it especially ideal for high-solubility, low-steric hindrance crosslinking in bioconjugation, PEGylation, and biomaterial applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
