Biotin-LC-NHS Ester
This product is a biotinylation reagent containing a long-chain (LC) spacer and an N-hydroxysuccinimide (NHS) ester. The NHS ester reacts efficiently with primary amines, while the LC spacer reduces steric hindrance. Therefore, it improves biotin accessibility in protein, peptide, and antibody labeling.
Key Features and Benefits
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NHS Ester Group: Reacts with primary amines (lysine side chains, N-termini) to form stable amide bonds.
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Long-Chain Spacer: In addition, enhances biotin accessibility by minimizing steric hindrance in labeled biomolecules.
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High Affinity Biotin Tag: Furthermore, binds strongly to streptavidin, avidin, and NeutrAvidin systems.
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Versatile Biotinylation: Suitable for proteins, peptides, oligonucleotides, and nanoparticles.
Applications
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Protein and Antibody Labeling: Biotinylates antibodies, enzymes, and other proteins for detection and purification.
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Affinity Capture: Also enables immobilization on streptavidin-coated beads, chips, or plates.
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Assay Development: Widely used in ELISA, Western blot, and immunohistochemistry.
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Nanotechnology: Finally, functionalizes nanoparticles and surfaces for biotin–streptavidin coupling.
Storage and Handling
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Storage: Store at –20 °C in a desiccated, light-protected container.
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Handling: Dissolve freshly in dry DMSO or DMF; avoid repeated freeze–thaw cycles.
Overall, this product combines efficient NHS ester reactivity with an extended spacer, providing superior biotinylation performance for proteins, probes, and assay development.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
