Benzoic acid, 4-[3-(trifluoromethyl)-3H-diazirin-3-yl] NHS

Benzoic acid, 4-[3-(trifluoromethyl)-3H-diazirin-3-yl] NHS

This product is a photo-reactive NHS ester crosslinker containing a diazirine group and a reactive N-hydroxysuccinimide ester. The diazirine enables UV-activated carbene insertion into nearby bonds, while the NHS ester couples efficiently with primary amines, making this reagent highly effective for covalent labeling and photoaffinity studies.

Key Features and Benefits:

• NHS Ester Group: Provides fast, efficient conjugation to primary amines.

• Diazirine Moiety: UV-activated carbene insertion enables covalent crosslinking.

• Trifluoromethyl Substitution: Enhances photoreactivity and stability.

• High Purity: Ensures reproducibility in photo-crosslinking and labeling experiments.

• Compact Design: Minimizes steric hindrance for efficient biomolecular interactions.

Applications:

• Photoaffinity Labeling: Covalently capture protein-ligand interactions under UV light.

• Bioconjugation: Link NHS-reactive biomolecules with photo-activatable groups.

• Drug Discovery: Map binding sites in receptor-ligand and enzyme-inhibitor systems.

• Proteomics Research: Identify protein-protein interactions with high specificity.

Storage and Handling:

• Storage: Store at –20 °C in a dry, light-protected container.

• Handling: Use freshly prepared anhydrous solutions (e.g., DMF, DMSO) for maximum stability.

This product combines amine-reactive chemistry with photo-crosslinking functionality, making it an especially versatile tool for mapping biomolecular interactions and advanced chemical biology research.

AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.