CM-DiI (CellTracker™ CM-DiI, CAS: 180854-97-1) is an orange-red fluorescent dye used for long-term cell membrane labeling. It integrates into lipid bilayers and forms covalent bonds with intracellular thiol-containing proteins, ensuring stable fluorescence in both live and fixed cells.
Key Features:
- Long-Term Labeling: Retains stable fluorescence for at least 72 hours, spanning multiple cell generations.
- Water Solubility: Offers better solubility than traditional DiI, simplifying preparation and use in aqueous environments.
- Fixation Compatibility: Stays intact after aldehyde-based fixation and permeabilization, making it suitable for immunohistochemistry and other post-fixation analyses.
- Fluorescence Specs: Excitation at ~553 nm and emission at ~570 nm, producing bright orange-red fluorescence.
Applications:
- Cell Tracking: Monitors cell movement, migration, and division in live-cell imaging.
- Tissue Staining: Labels cells in tissue sections for fluorescence microscopy.
- Neuronal Labeling: Traces neural pathways in neurobiology studies.
CM-DiI’s stable fluorescence, low cytotoxicity, and compatibility with fixation protocols make it essential for cell tracking and tissue imaging in biological research.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
